PoplarV1, Main, Exploration, bibRecord, 003306

Direct amplification of DNA from fresh and preserved ectomycorrhizal root tips.

Identifieur interne : 003306 ( Main/Exploration ); précédent : 003305; suivant : 003307

Direct amplification of DNA from fresh and preserved ectomycorrhizal root tips.

Auteurs : Elizabeth Bent [États-Unis] ; D Lee Taylor

Source :

Journal of microbiological methods [ 1872-8359 ] ; 2010.

RBID : pubmed:19963016

Descripteurs français

KwdFr :
- ADN fongique (isolement et purification), Manipulation d'échantillons (méthodes), Mycorhizes (croissance et développement), Mycorhizes (génétique), Méristème (microbiologie), Plantes (microbiologie), Réaction de polymérisation en chaîne (méthodes), Sensibilité et spécificité (MeSH).
MESH :
- croissance et développement : Mycorhizes.
- génétique : Mycorhizes.
- isolement et purification : ADN fongique.
- microbiologie : Méristème, Plantes.
- méthodes : Manipulation d'échantillons, Réaction de polymérisation en chaîne.
- Sensibilité et spécificité.

English descriptors

KwdEn :
- DNA, Fungal (isolation & purification), Meristem (microbiology), Mycorrhizae (genetics), Mycorrhizae (growth & development), Plants (microbiology), Polymerase Chain Reaction (methods), Sensitivity and Specificity (MeSH), Specimen Handling (methods).
MESH :
- chemical , isolation & purification : DNA, Fungal.
- genetics : Mycorrhizae.
- growth & development : Mycorrhizae.
- methods : Polymerase Chain Reaction, Specimen Handling.
- microbiology : Meristem, Plants.
- Sensitivity and Specificity.

Abstract

Methods are described by which DNA can be amplified directly from ectomycorrhizal root tip homogenates of a variety of plant species (Picea mariana (black spruce), Betula papyrifera (paper birch), Populus tremuloides (trembling aspen) and Alnus sp.(alder)), including root tips that have been preserved in RNA Later (Ambion, Austin, TX). In most cases for extracts and homogenates diluted 10-fold prior to PCR, and in all cases for 100-fold dilutions, direct amplification of DNA from fresh root tip homogenates yielded as many or more ng of PCR amplicon (fungal ITS region) than amplification of DNA extracted from the same tips using a commercial kit or a manual ethanol precipitation-based method. For alder root tip extracts diluted 10-fold, the commercial kit method yielded more ng of PCR amplicon than 10-fold diluted, although direct use of homogenates still resulted in amplification in all tips tested. We also demonstrate consistent amplification of DNA from homogenates of birch, spruce and aspen ectomycorrhizal root tips preserved for 4months in RNA Later.

DOI: 10.1016/j.mimet.2009.11.011
PubMed: 19963016

Affiliations:

États-Unis

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Le document en format XML

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<term>Mycorrhizae (growth & development)</term>
<term>Plants (microbiology)</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>Sensitivity and Specificity (MeSH)</term>
<term>Specimen Handling (methods)</term>
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<term>Manipulation d'échantillons (méthodes)</term>
<term>Mycorhizes (croissance et développement)</term>
<term>Mycorhizes (génétique)</term>
<term>Méristème (microbiologie)</term>
<term>Plantes (microbiologie)</term>
<term>Réaction de polymérisation en chaîne (méthodes)</term>
<term>Sensibilité et spécificité (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Fungal</term>
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<term>Specimen Handling</term>
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<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Méristème</term>
<term>Plantes</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Meristem</term>
<term>Plants</term>
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<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Manipulation d'échantillons</term>
<term>Réaction de polymérisation en chaîne</term>
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<keywords scheme="MESH" xml:lang="en"><term>Sensitivity and Specificity</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Methods are described by which DNA can be amplified directly from ectomycorrhizal root tip homogenates of a variety of plant species (Picea mariana (black spruce), Betula papyrifera (paper birch), Populus tremuloides (trembling aspen) and Alnus sp.(alder)), including root tips that have been preserved in RNA Later (Ambion, Austin, TX). In most cases for extracts and homogenates diluted 10-fold prior to PCR, and in all cases for 100-fold dilutions, direct amplification of DNA from fresh root tip homogenates yielded as many or more ng of PCR amplicon (fungal ITS region) than amplification of DNA extracted from the same tips using a commercial kit or a manual ethanol precipitation-based method. For alder root tip extracts diluted 10-fold, the commercial kit method yielded more ng of PCR amplicon than 10-fold diluted, although direct use of homogenates still resulted in amplification in all tips tested. We also demonstrate consistent amplification of DNA from homogenates of birch, spruce and aspen ectomycorrhizal root tips preserved for 4months in RNA Later.</div>
</front>
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<CopyrightInformation>Copyright  2009 Elsevier B.V. All rights reserved.</CopyrightInformation>
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Direct amplification of DNA from fresh and preserved ectomycorrhizal root tips.

Direct amplification of DNA from fresh and preserved ectomycorrhizal root tips.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki